AATCC TM 100: Antibacterial Finishes on Textile Materials: Assessment of

Purpose and Goals

Evaluate and compare the antimicrobial activity of multiple textiles in laboratory-controlled testing.

Testing

This testing will be performed according to the standard AATCC TM100. Circular textile swatches will be placed into a sterilized jar, inoculated with a liquid microbial suspension, and incubated at the test organism’s ideal growth temperature (such as 37°C) for 24 hours or a specified contact time. At the end of the contact time, the textile’s antimicrobial activity will be neutralized. The microbes remaining on the swatches will be recovered into a solution which will be dilution-plated on nutritive agar to provide quantifiable viable microbial burden data for each sample.
Testing will include the microbial strains:
- Klebsiella pneumoniae (ATCC 4352 standard), a Gram-negative bacteria.
- Staphylococcus aureus (ATCC 6538 standard), a Gram-positive bacteria.
- Pseudomonas aeruginosa (strain TBD), a Gram-negative bacteria.
Testing will include 24 total data points per microbial strain:
- 2 materials (1 test textiles and 1 provided/in-house negative control)
  - Note: Please provide at least 10 grams of each material per time-point.
- 2 time-points (0 and 24 hours)
- 3 replicates per sample at each test condition
- 2 independent tests
Note: Optional GLP services are available for an additional 25% fee.
Note: Bulk discounts are available for 4+ samples.

Data Analysis

In-house control results will be used to verify test validity.
Data will be calculated to determine the quantifiable microbial burden in colony-forming units per gram of textile material (CFU/g) remaining on each sample.
Data will be analyzed to determine the consistency between replicate samples in each set (n=6).
Data will be analyzed to determine the percent reductions and log reductions of microbial burdens on each/the test material compared to the negative control at each test condition.

Typical Turn Around Time