ISO 22196: Measurement of antibacterial activity on plastics and other non-porous surfaces

ISO 22196 – Measurement of antibacterial activity on plastics and other non-porous surfaces

https://www.iso.org/standard/54431.html

Purpose and Goals

  • Evaluate and compare the antimicrobial activity of non-porous materials in laboratory-controlled testing. 

Testing

  • This testing will be performed as a modification to the International Organization for Standardization Test method ISO 22196. 
  • Sections (dimensions to be determined) of the test material will be disinfected with an ethanol solution, inoculated with a liquid bacterial suspension, and covered with a sterile film to ensure a contact area of approximately 40mm x 40mm (or equivalent). The inoculated sections will be incubated for the specified time-point in a humidified chamber. After incubation, the bacteria from each section will be recovered into a neutralizing solution and then dilution-plated on nutritive agar to provide quantifiable viable bacterial burden data for each section tested.
  • Each test will include one of the microbial strains:
    • Staphylococcus aureus, a Gram-positive bacteria.
    • Escherichia coli, a Gram-negative bacteria.
    • Clostridioides difficile bacterial spores. 
  • Testing will include 24 total data points per microbial strain: 
    • 2 materials: 1 test materials and 1 provided/in-house negative control).
      • Note: Please provide at least 15,000 cm2 of each material per time-point. 
    • 2 time-points: 0 and 24 hours.
    • 3 replicates sections of each test material at each test condition
    • 2 independent tests
  • Note: Optional GLP services are available for an additional 25% fee.

Data Analysis

  • In-house control results will be used to verify test validity. 
  • Data will be calculated to determine the quantifiable microbial burden in colony-forming units per gram of textile material (CFU/g) remaining on each sample.
  • Data will be analyzed to determine the consistency between replicate samples in each set (n=6).
  • Data will be analyzed to determine the percent reductions and log reductions of microbial burdens on the/each test material compared to the negative control at each test condition. 

Typical Turn Around Time

  • (per strain) 2-3 weeks following receipt of the test materials