ISO 18184: Textiles — Determination of antiviral activity of textile products

Link: https://www.iso.org/standard/71292.html

Purpose and Goals

• Evaluate and compare the antiviral activity of multiple textiles in laboratory-controlled testing. 

Testing

• This testing will be a modification of ISO 18184. Textile swatches will be inoculated with a liquid viral suspension, incubated at room temperature and ambient relative humidity for a specified contact time, and then recovered into a solution which will be dilution-plated in cell cultures to provide quantifiable viable viral burden data for each swatch tested.
• Testing will include the viral strains:
  • Influenza A virus (H1N1) ATCC VR-1469
  • Human Coronavirus strain ​OC43​ ATCC VR-​1558 (a betacoronavirus like SARS-CoV-2)
  • Feline calicivirus (FCV) strain F-9 ATCC VR-782 (a surrogate for human norovirus)
• Testing will include 72 total data points: 
  • 4 materials (3 test textiles and 1 provided/in-house negative control)
    • Note: Please provide at least 5 grams of each material per time-point. 
  • 6 time-points (0, 1 hour, 2 hours, 4 hours, 12 hours, and 24 hours)
  • 3 replicate swatches at each test condition
• Note: Optional GLP services are available for an additional 25% fee.

Data Analysis

• In-house control results will be used to verify test validity. 
• Data will be calculated to determine the quantifiable viral burden in plaque- or foci-forming units per disc (PFU/disc or FFU/disc) remaining on each test location.
• Data will be analyzed to determine the consistency between replicate swatches.
• Data will be analyzed to determine the percent reductions and log reductions of viral burdens on each/the test material compared to the provided negative control at each test condition. 

Typical Turn Around Time

• (per strain) 6-8 weeks following receipt of the test materials