Purpose and Goals
- Evaluate and compare the antimicrobial efficacies of a novel disinfection device against a variety of microbes on different test locations in laboratory-controlled testing.
Materials and Methods
- This testing will be performed per the US EPA’s MB-06-09, Standard Operating Procedure for Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica.
- Glass slide carriers (approx. 25x25mm) will be inoculated with a liquid microbial suspension and dried. Microbes from each control carrier will be recovered into a solution which will be dilution-plated to provide quantifiable viable microbial burden data for each control carrier.
- Treated carriers will be sprayed with the disinfectant product per the manufacturer’s instructions. After the indicated contact time, the microbes from each treated carrier will be recovered into a culture tube of neutralizing nutrient solution (and a secondary sub-culture tube if necessary for complete neutralization) which will be incubated to provide qualitative growth/no growth data for each treated carrier by evaluation of turbidity in the solution. If the disinfectant product passes the performance standard, 20% of the no-growth culture tubes will be sub-cultured on nutrient agar to confirm results.
- This testing will include 5% organic soil (serum) in the inoculum solution.
- Each test will include one microbial strain:
- Staphylococcus aureus ATCC 6538 bacteria,
- Pseudomonas aeruginosa ATCC 15442 bacteria,
- Salmonella enterica ATCC 10708 bacteria,
- Methicillin-resistant Staphylococcus aureus ATCC 33592 (MRSA) bacteria,
- Clostridioides difficile ATCC BAA-1870 or 43598 (formerly known as Clostridium difficile) bacterial spores, or
- Feline calicivirus (FCV) strain F-9 ATCC VR-782 (a surrogate for human norovirus).
- Each test will include 80 carriers to ensure sufficient materials for:
- 60 test carriers (minimum)
- 6 unexposed control carriers (3 recovered before testing the treated carriers, and 3 recovered after testing is complete)
- 1 viability control carrier
- Each test will include 1 exposure time: TBD minutes.
- Note: Optional GLP services are available for an additional 25% fee.
- Data for the control carriers will be calculated to determine the quantifiable bacterial/fungal burden in colony-forming units per carrier (CFU/carrier) or viral burden in plaque- or foci-forming units per carrier (PFU/carrier or FFU/carrier) remaining on each test carrier.
- Data will be analyzed to determine the consistency between replicate control carriers.
- Treated carriers will be reported as growth / no-growth only.
Typical Turn Around Time
- Bacterial/fungal (per strain): 4-6 weeks following receipt of the test materials.
- Viral (per strain): 6-8 weeks following receipt of the test materials.