ISO 18184: Textiles — Determination of antiviral activity of textile products

Purpose and Goals

Evaluate and compare the antiviral activity of multiple textiles in laboratory-controlled testing.

Testing

Textile swatches will be inoculated with a liquid viral suspension, incubated at room temperature and ambient relative humidity for a specified contact time, and then recovered into a solution which will be dilution-plated in cell cultures to provide quantifiable active viral burden data for each swatch tested.
Each test will include one of the viral strains:
- Human Coronavirus strain OC43 (HCoV-OC43) (ATCC VR-1558), an enveloped RNA betacoronavirus like the recent pandemic strain SARS-CoV-2.
- Human Coronavirus strain 229E (HCoV-229E) (ATCC VR-740), an enveloped RNA alphacoronavirus.
- Human Coronavirus strain NL63 (HCoV-NL63) (NIAID-BR NR-470), an enveloped RNA alphacoronavirus.
- Influenzavirus A (H1N1) (ATCC VR-1469), an enveloped RNA virus.
- Influenzavirus A (H3N2) (ATCC VR-1680), an enveloped RNA virus.
- Feline Calicivirus (FCV) strain F-9 (ATCC VR-782), a non-enveloped RNA virus laboratory surrogate for human norovirus.
Testing will include 8 total data points:
- 2 total materials (such as 1 test textile and 1 in-house negative control)
  - Note: Please provide at least 5 grams of each material per time-point.
- 2 time-points (such as 0 and 2 hours)
- 2 replicate swatches at each test condition
Note: Optional GLP services are available for an additional fee.

Data Analysis

In-house control results will be used to verify test validity.
Data will be calculated to determine the quantifiable viral burden in plaque- or foci-forming units per disc (PFU/disc or FFU/disc) remaining on each test location.
Data will be analyzed to determine the consistency between replicate swatches.
Data will be analyzed to determine the percent reductions and log reductions of viral burdens on each/the test material compared to the provided negative control at each test condition.

Typical Turn Around Time